Plant Phenotyping At
Early Developmental Stages
High-throughput seedlings phenotyping represent a technological leverage MIAtecs can help you to achieve using the last imaging and image processing methods.
Let's dive together into the pivotal seedling growth phases, where critical morphological and developmental choices shape the future.
Simultaneous analysis of up to 20 seedlings in time.
Development of specific algorithms.
APPLICATIONS
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Developmental biology
Analysis of the apical hook opening mechanisms.
Biosensors imaging
pH imaging
Apoplastic pH in plants can be imaged using the Apo-pHusion biosensor (among others) expressed throughout the whole seedling (Gjetting et al., 2012). This allows for the observation of distinct acidic and basic pH zones along the seedling and enables tracking of apoplastic pH dynamics during plant growth, both in wild-type plants and in mutants affected in cellulose biosynthesis.
WT Apo-pHusion in WT Apo-pHusion in mutants
Calcium imaging
Chemical imaging is non-destructive and enables real-time, quantitative, and reliable measurement of chemical compounds. In this case, calcium imaging was performed using the RGeco-1 biosensor (Zhao et al., 2011), allowing for the detection and analysis of Ca²⁺ spikes in the root tip.