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Particle or object tracking have been made possible in the recent years due to the availability of video microscopy and powerful algorithms, but also to the fast development of online storage and computing tools.

With object tracking, all of your conventional analyses are bring to a higher level, allowing you to extract information that are not accessible without in vivo imaging.

Cellulose Synthase Complexes (CESA) trafficking

The plasma membrane represents a cellular compartment with a role of barrier between external and internal physiological events.

In this project, we have tracked proteins organized in complexes called CESA, specialized in the cellulose biosynthesis. The goal of this image processing development using spinning disk microscopy is to be able to track CESA complexes and characterize they dynamics and behaviour in different mutants background or under different chemical treatments.

Traits computed

  • CESAs speed

  • CESAs trajectories

  • CESAs signal intensity

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Nucleus-targeted peptides tracking during Arabidopsis cross opening

Plant peptides are proteins which play a key role in the plant growth and development and in plant immunity.

In this time-lapse sequence we can observe the expression of a peptide targeted in the nucleus of a growing Arabidopsis seedlings using a simple widefield epifluorescence microscope.

Traits computed

  • Nucleus speed

  • Nucleus trajectories

  • Nucleus signal intensity

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