top of page

Most of the biological processes that occur in mammalian and plant cells are controlled by chemical signalling.

The analysis of those chemical signals usually relies on traditionnal chemical measurements with extraction, purification, identification and analysis.

At the opposite, chemical imaging is non-destructive and allows for real-time, quantitative and reliable chemical product measurements.

Calcium signalling

Here, calcium imaging has been performed using RGeco-1 biosensor
(Zhao et al., 2011).
The signal is analyzed in the root tip where we can detect Ca²+ spikes.

Traits computed

  • Ca²+ spikes counting

  • Ca²+ spikes tracking and characterization

ZOOM

pH Imaging

pH in the plant apoplast can be imaged using Apo-pHusion biosensor expressed in the whole seedling (Getting et al., 2012).
We can observe several acidic and basics pH areas along the seedling and
track the apoplastic pH dynamics during plant growth simultaneously in WT and mutants affected in the cellulose biosynthesis.

Traits computed

Relative pH values along the whole seedling

WT                   Apo-Phusion in WT              Apo-pHusion in mutants

zdzdzd.PNG

Quantitative analysis (maps) of relative apoplastic pH values along the whole seedling

bottom of page