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Image processing is often performed on 2D RGB images. However, the last laser scanning microscopes coupled with live cell imaging and genetically modified organisms expressing several fluorophores or chemical stainings require to push the limits of image processing.

Indeed, processing 6 dimentionnal datasets (x,y,z,t,channels, multiposition) requires particular skills MIAtecs can deal with.

3D detailed cell reconstruction

When imaging complexe processes, it is necessary to observe them in 3D at an exploratory way. This is often time consuming and also requires special skills.

In this example, the plasma membrane of the cell is represented in red, the microtubules in blue, the CESA3-GFP vesicules in yellow and the new developing plasma membrane at the cell plate in strong red and green.

The two movies are from our previous work, in collaboration with Samantha Vernhettes (Primary Cell Wall team, IJPB, Versailles) - Published in Plant Journal, 2013 : https://doi.org/10.1111/tpj.12362

Traits computed in 3D

  • Cellular compartments reconstruction and modelisation

  • Quantitative analysis in 3 dimensions

  • Mean representation and normalisation

6D imaging of a pH biosensor (Apo-pHusion) in Arabidopsis stem

When working on complexe topics, such as pH imaging in the apoplast, the best strategy consists into image entire pieces of tissue. This can be called exploratory imaging.

In this example, we can observe the apoplastic pH evolution in 3D. Image processing allows to reconstruct the images stack to better visualize the pH view different point of views.

Traits computed in 6D

  • Tissue reconstruction

  • Ratiometric signal (eGFP + mRFP) reconstruction

  • Signal analyses

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